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Captain Matt Lawrence starts a three-match ban for his sending-off in last weekend's defeat at Coventry while goalkeeper Andy Marshall (knee), defender Jamie Vincent (calf) and forwards Carlos Fangueiro (ankle), Barry Hayles (knee) and Carl Asaba (back) are all unavailable. Jermaine Wright and Phil Ifil have also returned to their parent clubs after completing their loan spells.
However, Lee does welcome back striker Ben May after illness and Mark Phillips is fit again after injury.
"We will have to shuffle the pack yet again for Reading," said Lee.
"We were already short of bodies after losing the loan players, and the situation seems to get worse by the day."